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国内にHTRF®専用ラボを設置

HTRF®専用ラボを事務所に併設し、カスタム標識やアッセイ系の構築等に対応できる体制を整えています。経験豊富な専任技術者が 皆様からのお問い合わせにお答えします。

タグ付き分子限定分子間相互作用確認受託サービス

HTRF®のメリットを受託で体験してみませんか?

ELISAやWestern Blottingと比較しても多くのメリットがあるHTRF®法で、ご自身のサンプルでの分子間相互作用を確認してみたいけど、測定機器が手元にない・・・或いは、初めての手法だから操作に不安がある・・・と躊躇されていらっしゃる方に朗報です!

新メニュー登場!

タンパク – タンパク間、タンパク – ペプチド間、タンパク – 核酸間などの相互作用やプロテアーゼの活性測定など、実際にアッセイ系を構築することは簡単ではありません。弊社は熟練した技術者がHTRF®法に関して永年蓄積してきた知識、ノウハウを駆使して、上記のような実験に対して最適なHTRF®アッセイを構築します。お客様のお手元にある、タグ付き(※メニューにより条件が異なります。)分子をご提供いただければ、弊社の国内ラボにて作業を行い、組み合わせ試験データや至適化アッセイ条件等を書面レポートにてご報告致します。

サンプルの条件さえ合えば・・・製品を買うより安い!!

この新規メニューの特徴は、弊社の製品ラインナップであるToolbox 試薬に、お客様のサンプルに導入されているタグを認識するものがあれば、ご自身ですべての試薬をご購入されるよりも安価に分子間相互作用を確認できることです。具体的にどれくらいお得か?の具体例を下記に示します。なお、お返しするデータを元に、弊社で構築したアッセイ系でさらにご研究を進めたい場合は、該当するToolbox 試薬もしくはカスタムラベリングをお求めください。

進め方について

  • お申し込みは弊社代理店を経由してお願い致します。代理店がお渡しする申し込み用紙に必要事項をご記入の上、代理店担当者にお渡しください。尚、該当代理店のご確認は、お手数ですが弊社営業部043-306-8712またはhtrf@xxxxx.jp(xxxxにはcisbioを代入ください)までお問い合わせください。
  • お客様が望まれるアッセイ系の規格詳細について、先ず弊社技術者が打合せをさせていただきます。
  • アッセイ系構築に必要となるサンプル(例:抗体の場合、100~200μg; 但しBSAおよび担体を含まず)は元払いで送付ください。
  • 実験終了後、得られた詳細データとアッセイ条件等を示すレポートを書面で提出します。未標識サンプルは作業終了後に返却致します。
  • お預かりしたサンプル間の結合や活性の確認を100% 保証するものではないことを予めご了承ください。

両側タグ付きコースの場合

  • アッセイ例:タンパク質A -GSTタグとタンパク質B ‒biotinとの相互作用
  • 応用例:タンパク質 – タンパク質相互作用、プロテアーゼ活性測定等
  • 使用するHTRF 試薬:Toolbox 試薬のみ
  • Toolbox 試薬(4 種類まで)
  • アッセイ構築に必要なタンパク質、ペプチド、抗体等の材料をご提供いただきます
  • 組み合わせ試験の実施
  • 書面レポートの提出
  • 期間:作業開始後、約3 週間

片側タグ付きコースの場合

  • アッセイ例:タンパク質A -GSTタグとタンパク質B との相互作用
  • 応用例:タンパク質 – タンパク質相互作用、プロテアーゼ活性測定等
  • 使用するHTRF 試薬:Toolbox 試薬及びカスタムラベル
  • Toolbox 試薬(2 種類まで)
  • タグのない分子のカスタムラベリング作業(2 件まで)
  • アッセイ構築に必要なタンパク質、ペプチド、抗体等の材料をご提供いただきます
  • 組み合わせ試験の実施
  • 書面レポートの提出
  • 期間:作業開始後、約1ヶ月

 

【お問合せ先】
シスバイオ株式会社
043-306-8712
email: htrf@xxxxx.jp(xxxxにはcisbioを代入ください)

日本発のHTRF®論文

【GPCR】


〇使用キット・試薬; cAMP
Contribution of active and inactive states of the human 5-HT4d receptor to the functional activities of 5-HT4-receptor agonists
J Pharmacol Sci. 2008 Jul;107(3):251-9
https://www.ncbi.nlm.nih.gov/pubmed/18587219


〇使用キット・試薬; cAMP
Pharmacological Profile of Lurasidone, a Novel Antipsychotic Agent with Potent 5-HT7 and 5-HT1A Receptor Activity
Journal of Pharmacology and Experimental Therapeutics July 2010, 334 (1) 171-181
https://www.ncbi.nlm.nih.gov/pubmed/20404009


〇使用キット・試薬; cAMP
Medium-chain Fatty Acid-sensing Receptor, GPR84, is a Proinflammatory Receptor
J Biol Chem. 2013 Apr 12;288(15):10684-91
https://www.ncbi.nlm.nih.gov/pubmed/23449982


〇使用キット・試薬; cAMP
Neurophysiologic and Antipsychotic Profiles of TASP0433864, a Novel Positive Allosteric Modulator of Metabotropic Glutamate 2 Receptor
J Pharmacol Exp Ther. 2014 Dec;351(3):642-53
https://www.ncbi.nlm.nih.gov/pubmed/25277141


〇使用キット・試薬; cAMP, IP-One, insulin
Novel Zn2+ Modulated GPR39 Receptor Agonists Do Not Drive Acute Insulin Secretion in Rodents
PLoS One. 2015 Dec 31;10(12):e0145849
https://www.ncbi.nlm.nih.gov/pubmed/26720709


〇使用キット・試薬; cAMP
Study on Physiological Roles of Stimulation of Prostaglandin E2 Receptor Subtype EP2 in Urethral Function in Rats
Low Urin Tract Symptoms. 2016 May;8(2):125-9.
https://www.ncbi.nlm.nih.gov/pubmed/27111625


〇使用キット・試薬; cAMP
Synthesis of both enantiomers of 1,2,3,4-tetrahydroisoquinoline derivative IPPAM-1 and enantio-dependency of its positive allosteric modulation of prostacyclin receptor
Bioorganic & Medicinal Chemistry Letters, Available online 29 March 2017
http://www.sciencedirect.com/science/article/pii/S0960894X17303335


〇使用キット・試薬; cAMP
Rho kinase-dependent desensitization of GPR39; a unique mechanism of GPCR downregulation
Biochemical pharmacology , 2017 , (140):105-114
http://www.ncbi.nlm.nih.gov/pubmed/28619258


〇使用キット・試薬; cAMP
Effects of 4(1H)-quinolinone derivative, a novel non-nucleotide allosteric purinergic P2Y2 agonist, on cardiomyocytes in neonatal rats
Sci Rep. 2017 Jul 20;7(1):6050
https://www.ncbi.nlm.nih.gov/pubmed/28729619


【Protein-Protein Interaction reagents】


〇使用キット・試薬; PT66-Eu, SA-XL
Development of a Homogeneous Time-Resolved Fluorescence Assay for High Throughput Screening to Identify Lck Inhibitors: Comparison with Scintillation Proximity Assasy and Streptavidin-Coated Plate Assay
J Biomol Screen. 2000 Dec;5(6):463-70
https://www.ncbi.nlm.nih.gov/pubmed/11598464


〇使用キット・試薬; anti-DNP-XL, SA-Eu
A Homogeneous Caspase-3 Activity Assay Using HTRF Technology
J Biomol Screen. 2002 Jun;7(3):267-74
https://www.ncbi.nlm.nih.gov/pubmed/12097189


〇使用キット・試薬; Eu cryptate
Development of high-throughput spermidine synthase activity assay using homogeneous time-resolved fluorescence
Anal Biochem. 2006 Apr 15;351(2):229-40
https://www.ncbi.nlm.nih.gov/pubmed/16472757


〇使用キット・試薬; anti-GST-Eu, SA-XL
Unique Properties of Coactivator Recruitment Caused by Differential Binding of FK614, an Anti-diabetic Agent, to Peroxisome Proliferator Activated Receptor g
Biol Pharm Bull. 2006 Mar;29(3):423-9.
https://www.ncbi.nlm.nih.gov/pubmed/16508139


〇使用キット・試薬; PT66-Eu, anti-6HIS-XL
E6201[(3S,4R,5Z,8S,9S,11E)-14-(ethylamino)-8,9,16-trihydroxy-3,4-dimethyl-3,4,9,19-tetrahydro-1H-2-benzoxacyclotetradecine-1,7(8H)-dione], a novel kinase inhibitor of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK)-1 and MEK kinase-1: in vitro characterization of its anti-inflammatory and antihyperproliferative activities
J Pharmacol Exp Ther. 2009 Nov;331(2):485-95
https://www.ncbi.nlm.nih.gov/pubmed/19684251


〇使用キット・試薬; anti-FLAG-Eu, anti-6HIS-XL
A homogeneous time-resolved fluorescence-based high-throughput screening system for discovery of inhibitors of IKKb–NEMO interaction
Anal Biochem. 2010 Oct 1;405(1):19-27
https://www.ncbi.nlm.nih.gov/pubmed/20522330


〇使用キット・試薬; anti-GST-Eu, SA-XL
Pharmacology and in vitro profiling of a novel peroxisome proliferator-activated receptor γ ligand, Cerco-A
Biol Pharm Bull. 2011;34(7):1094-104
https://www.ncbi.nlm.nih.gov/pubmed/21720019


〇使用キット・試薬; anti-GST-Eu, SA-XL
Direct in Vitro and in Vivo Evidence for Interaction between Hsp47 Protein and Collagen Triple Helix
J Biol Chem. 2012 Feb 24;287(9):6810-8
https://www.ncbi.nlm.nih.gov/pubmed/22235129


〇使用キット・試薬; anti-6HIS-Eu, SA-XL
Antitumor Activity and Pharmacodynamic Biomarkers of a Novel and Orally Available Small-Molecule Antagonist of Inhibitor of Apoptosis Proteins
Mol Cancer Ther. 2013 Feb;12(2):230-40
https://www.ncbi.nlm.nih.gov/pubmed/23243058


〇使用キット・試薬; anti-H3K9me0-Eu, SA-XL665
JMJD1A is a signal-sensing scaffold that regulates acute chromatin dynamics via SWI/SNF association for thermogenesis
Nat Commun. 2015 May 7;6:7052. doi: 10.1038/ncomms8052
https://www.ncbi.nlm.nih.gov/pubmed/25948511


〇使用キット・試薬; anti-GST-Tb
An HTRF based high-throughput screening for discovering chemical compounds that inhibit the interaction between Trypanosomabrucei Pex5p and Pex14p
Biochemistry and Biophysics Reports 6, (2016) 260–265
https://www.ncbi.nlm.nih.gov/pubmed/28955883


〇使用キット・試薬; Streptavidin
K-Ras(G12D)-selective inhibitory peptides generated by random peptide T7 phage display technology.
Biochemical and biophysical research communications , 2017 , 484 ( 3 ): 605-611
http://www.ncbi.nlm.nih.gov/pubmed/28153726


〇使用キット・試薬; anti-FLAG
Discovery of a novel prolyl-tRNA synthetase inhibitor and elucidation of its binding mode to the ATP site in complex with l-proline
Biochemical and biophysical research communications , 2017 , 488 ( 2 ): 393-399
http://www.ncbi.nlm.nih.gov/pubmed/28501621


〇使用キット・試薬; Streptavidin
Universal and Quantitative Method To Evaluate Inhibitor Potency for Cysteinome Proteins Using a Nonspecific Activity-Based Protein Profiling Probe
Biochemistry , 2017 , 56(23):2921-2927
http://www.ncbi.nlm.nih.gov/pubmed/28520393


〇使用キット・試薬; anti-6HIS-Eu, anti-GST-XL
Identification of claudin-4 binder that attenuates tight junction barrier function by TR-FRET-based screening assay
Sci Rep. 2017 Nov 6;7(1):14514
https://www.ncbi.nlm.nih.gov/pubmed/29109448


〇使用キット・試薬; anti-FLAG-Eu, SA-d2
Novel small molecule inhibiting CDCP1-PKCδ pathway reduces tumor metastasis and proliferation
Cancer Sci. 2017 May;108(5):1049-1057
https://www.ncbi.nlm.nih.gov/pubmed/28256037

【Enzyme assay & Biomarker】


〇使用キット・試薬; anti-DNP-XL, SA-Eu
A Homogeneous Caspase-3 Activity Assay Using HTRF Technology
J Biomol Screen. 2002 Jun;7(3):267-74
https://www.ncbi.nlm.nih.gov/pubmed/12097189




〇使用キット・試薬; cortisol
Sterenin A, B, C and D, Novel 11 b b -Hydroxysteroid Dehydrogenase Type 1 Inhibitors from Stereumsp. SANK 21205
J. Antibiot. 61(3): 128–135, 2008
https://www.ncbi.nlm.nih.gov/pubmed/18503190


〇使用キット・試薬; cortisol
HIS-388, a novel orally active and long-acting 11β-hydroxysteroid dehydrogenase type 1 inhibitor, ameliorates insulin sensitivity and glucose intolerance in diet-induced obesity and nongenetic type 2 diabetic murine models
J Pharmacol Exp Ther. 2014 Oct;351(1):181-9
https://www.ncbi.nlm.nih.gov/pubmed/25100752

 




〇使用キット・試薬; anti-H3K9me0-Eu,SA-XL665
JMJD1A is a signal-sensing scaffold that regulates acute chromatin dynamics via SWI/SNF association for thermogenesis
Nat Commun. 2015 May 7;6:7052. doi: 10.1038/ncomms8052
https://www.ncbi.nlm.nih.gov/pubmed/25948511


〇使用キット・試薬; EPIgeneous Methyltransferase Assay
Impact of brown rice-specific γ-oryzanol on epigenetic modulation of dopamine D2 receptors in brain striatum in high-fat-diet-induced obesity in mice
Diabetologia , 2017;60(8):1502-1511
http://www.ncbi.nlm.nih.gov/pubmed/28528402


〇使用キット・試薬; EPIgeneous Methyltransferase Assay
Identification of AHCY inhibitors using novel high-throughput mass spectrometry
Biochemical and biophysical research communications , 2017 , 9;491(1):1-7
http://www.ncbi.nlm.nih.gov/pubmed/28533090


〇使用キット・試薬; EPIgeneous Methyltransferase Assay
Aristeromycin and DZNeP cause growth inhibition of prostate cancer via induction of mir-26a
European journal of pharmacology , 2017 ,(812):138-146:
http://www.ncbi.nlm.nih.gov/pubmed/28705714


〇使用キット・試薬; cGMP
Green Tea Polyphenol EGCG Upregulates Tollip Expression by Suppressing Elf-1 Expression
J Immunol. 2017 Nov 1;199(9):3261-3269
https://www.ncbi.nlm.nih.gov/pubmed/28954885


〇使用キット・試薬; cGMP
Sustained activation of guanylate cyclase-A with TDT, a natriuretic peptide derivative, exhibits cardiorenal protection in Dahl salt-sensitive hypertensive rats
Journal of Pharmacology and Experimental Therapeutics December 2017, 363 (3) 402-410
https://www.ncbi.nlm.nih.gov/pubmed/29021382


【Diabetes & Obesity】

 


〇使用キット・試薬; insulin
Actin dynamics regulated by the balance of neuronal Wiskott-Aldrich syndrome protein (N-WASP) and cofilin activities determines the biphasic response of glucose-induced insulin secretion
J Biol Chem. 2013 Sep 6;288(36):25851-64
https://www.ncbi.nlm.nih.gov/pubmed/23867458


〇使用キット・試薬; insulin
Glutamate Acts as a Key Signal Linking Glucose Metabolism to Incretin/cAMP Action to Amplify Insulin Secretion
Cell Rep. 2014 Oct 23;9(2):661-73
https://www.ncbi.nlm.nih.gov/pubmed/25373904


〇使用キット・試薬; cortisol
HIS-388, a novel orally active and long-acting 11β-hydroxysteroid dehydrogenase type 1 inhibitor, ameliorates insulin sensitivity and glucose intolerance in diet-induced obesity and nongenetic type 2 diabetic murine models
J Pharmacol Exp Ther. 2014 Oct;351(1):181-9
https://www.ncbi.nlm.nih.gov/pubmed/25100752


〇使用キット・試薬; insulin
Long-Term Pancreatic Beta Cell Exposure to High Levels of Glucose but Not Palmitate Induces DNA Methylation within the Insulin Gene Promoter and Represses Transcriptional Activity
PLoS ONE10(2):e0115350. doi:10.1371/journal.pone.0115350
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0115350


〇使用キット・試薬; insulin
Mice Deficient in Proglucagon-Derived Peptides Exhibit Glucose Intolerance on a High-Fat Diet but Are Resistant to Obesity
PLoS One. 2015 Sep 17;10(9):e0138322
https://www.ncbi.nlm.nih.gov/pubmed/26378455


〇使用キット・試薬; insulin, cAMP ,IP-One
Novel Zn2+ Modulated GPR39 Receptor Agonists Do Not Drive Acute Insulin Secretion in Rodents
PLoS One. 2015 Dec 31;10(12):e0145849
https://www.ncbi.nlm.nih.gov/pubmed/26720709


〇使用キット・試薬; ApoB
3D spheroid cultures improve the metabolic gene expression profiles of HepaRG cells.
Biosci Rep. 2015 May 7;35(3).
https://www.ncbi.nlm.nih.gov/pubmed/26182370


〇使用キット・試薬; insulin
Secreted factors from dental pulp stem cells improve glucose intolerance in streptozotocin-induced diabetic mice by increasing pancreatic β-cell function
BMJ Open Diabetes Res Care. 2015 Oct 19;3(1):e000128
https://www.ncbi.nlm.nih.gov/pubmed/26504525


〇使用キット・試薬; insulin
Role of Epac2A/Rap1 Signaling in Interplay Between Incretin and Sulfonylurea in Insulin Secretion
Diabetes. 2015 Apr;64(4):1262-72
https://www.ncbi.nlm.nih.gov/pubmed/25315008


〇使用キット・試薬; anti-H3K9me0-Eu, SA-XL665
JMJD1A is a signal-sensing scaffold that regulates acute chromatin dynamics via SWI/SNF association for thermogenesis
Nat Commun. 2015 May 7;6:7052. doi: 10.1038/ncomms8052
https://www.ncbi.nlm.nih.gov/pubmed/25948511


〇使用キット・試薬; insulin
Characterization of the Prediabetic State in a Novel Rat Model of Type 2 Diabetes, the ZFDM Rat
J Diabetes Res. 2015;2015:261418
https://www.ncbi.nlm.nih.gov/pubmed/25961052


〇使用キット・試薬; insulin
Endogenous GIP ameliorates impairment of insulin secretion in proglucagon-deficient mice under moderate beta cell damage induced by streptozotocin
Diabetologia (2016) 59:1533–1541
https://www.ncbi.nlm.nih.gov/pubmed/27053237


〇使用キット・試薬; insulin
A Novel Diphenylthiosemicarbazide Is a Potential Insulin Secretagogue for Anti-Diabetic Agen
PLoS One. 2016 Oct 20;11(10):e0164785. doi: 10.1371
https://www.ncbi.nlm.nih.gov/pubmed/27764176


〇使用キット・試薬; glucagon
Protein kinase C-d signaling regulates glucagon secretion from pancreatic islets
J. Med. Invest. 64 : 122 -128, February, 2017
http://medical.med.tokushima-u.ac.jp/jmi/vol64/pdf/v64_n1-2_p122.pdf


〇使用キット・試薬; EPIgeneous Methyltransferase Assay
Impact of brown rice-specific γ-oryzanol on epigenetic modulation of dopamine D2 receptors in brain striatum in high-fat-diet-induced obesity in mice
Diabetologia , 2017;60(8):1502-1511
http://www.ncbi.nlm.nih.gov/pubmed/28528402


〇使用キット・試薬; insulin
Essential roles of aspartate aminotransferase 1 and vesicular glutamate transporters in β-cell glutamate signaling for incretin-induced insulin secretion
PLoS One. 2017 Nov 1;12(11):
https://www.ncbi.nlm.nih.gov/pubmed/29091932

 

【Inflammation & Cytokine】
〇使用キット・試薬; human IL-17
Structural Basis of Digoxin That Antagonizes RORγt Receptor Activity and Suppresses Th17 Cell Differentiation and Interleukin (IL)-17 Production
J Biol Chem. 2011 Sep 9;286(36):31409-17
https://www.ncbi.nlm.nih.gov/pubmed/21733845


〇使用キット・試薬; human IL-1β, IL-6, IL-8, TNF-α
Synovial joint fluid cytokine levels in hip disease.
Rheumatology (Oxford). 2014 Jan;53(1):165-72
https://www.ncbi.nlm.nih.gov/pubmed/24136066


〇使用キット・試薬; PGE2, human IL-1β, IL-6, IL-8, TNF-α
Cyclic compressive loading on 3D tissue of human synovial fibroblasts upregulates prostaglandin E2 via COX-2 production without IL-1β and TNF-α
Bone Joint Res. 2014 Sep;3(9):280-8
https://www.ncbi.nlm.nih.gov/pubmed/25237168


〇使用キット・試薬; human IL-1β, IL-6, IL-8
Effect of platelet-rich plasma on proliferation of human synovial cells
J Osaka Dent Univ 2016(April); 50(1):31−35.
https://www.jstage.jst.go.jp/article/jodu/50/1/50_31/_pdf


〇使用キット・試薬; human IFN-γ, IL-17
Oral administration of a novel RORγt antagonist attenuates psoriasis-like skin lesion of two independent mouse models through neutralization of IL-17
J Dermatol Sci. 2017 Jan;85(1):12-19
https://www.ncbi.nlm.nih.gov/pubmed/27726924


〇使用キット・試薬; mouse IL-1α, IL-1β
Brain Interleukin-1 Facilitates Learning of a Water Maze Spatial Memory Task in Young Mice
Front Behav Neurosci. 2017 Oct 23;11:202. doi: 10.3389
https://www.ncbi.nlm.nih.gov/pubmed/29123474


〇使用キット・試薬; human IL-8
D-serine, a novel uremic toxin, induces senescence in human renal tubular cells via GCN2 activation
Scientific reports , 2017 , 7 ( 1 ): 11168
http://www.ncbi.nlm.nih.gov/pubmed/28894140


【Cancer & Kinase】


〇使用キット・試薬; PT66-Eu, SA-XL
Development of a Homogeneous Time-Resolved Fluorescence Assay for High Throughput Screening to Identify Lck Inhibitors: Comparison with Scintillation Proximity Assasy and Streptavidin-Coated Plate Assay
J Biomol Screen. 2000 Dec;5(6):463-70
https://www.ncbi.nlm.nih.gov/pubmed/11598464


〇使用キット・試薬; PT66-Eu, anti-6HIS-XL
E6201[(3S,4R,5Z,8S,9S,11E)-14-(ethylamino)-8,9,16-trihydroxy-3,4-dimethyl-3,4,9,19-tetrahydro-1H-2-benzoxacyclotetradecine-1,7(8H)-dione], a novel kinase inhibitor of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK)-1 and MEK kinase-1: in vitro characterization of its anti-inflammatory and antihyperproliferative activities.
J Pharmacol Exp Ther. 2009 Nov;331(2):485-95
https://www.ncbi.nlm.nih.gov/pubmed/19684251


〇使用キット・試薬; KinEASE TK
The selective anaplastic lymphoma receptor tyrosine kinase inhibitor ASP3026 induces tumor regression and prolongs survival in non-small cell lung cancer model mice
Mol Cancer Ther. 2014 Feb;13(2):329-40
https://www.ncbi.nlm.nih.gov/pubmed/24419060


〇使用キット・試薬; Streptavidin
K-Ras(G12D)-selective inhibitory peptides generated by random peptide T7 phage display technology
Biochemical and biophysical research communications , 2017 , 484 ( 3 ): 605-611
http://www.ncbi.nlm.nih.gov/pubmed/28153726


〇使用キット・試薬; EPIgeneous Methyltransferase Assay
Aristeromycin and DZNeP cause growth inhibition of prostate cancer via induction of mir-26a
http://www.ncbi.nlm.nih.gov/pubmed/28705714


〇使用キット・試薬; KinEASE TK
Gilteritinib, a FLT3/AXL inhibitor, shows antileukemic activity in mouse models of FLT3 mutated acute myeloid leukemia
Invest New Drugs (2017) 35:556–565
https://www.ncbi.nlm.nih.gov/pubmed/28516360


〇使用キット・試薬; anti-FLAG-Eu, SA-d2
Novel small molecule inhibiting CDCP1-PKCδ pathway reduces tumor metastasis and proliferation
Cancer Sci. 2017 May;108(5):1049-1057
https://www.ncbi.nlm.nih.gov/pubmed/28256037

 

日本発のHTRF®ポスター

・A direct cell-based assay for cortisol
C. Tokuda, J. Ouled Diaf, L. Jacquemart, E.J. Claret, P. Seguin
9th Annual meeting of the Society for Bimolecular Sciences (2003)

・IC50 determination of Carbenoxolone and Glycyrrhetinic acid on 11-beta hydroxysteroid dehydrogenase type 1 activity by HTRF®
C. Tokuda , J. Ouled Diaf , F. Chevallier, L. Jacquemart, M. Préaudat, P. Seguin
10th Annual meeting of the Society for Bimolecular Sciences (2004)

・ Development of a homogeneous heparanase assay using HTRF® technology
C. Tokuda , L. Jacquemart, M. Préaudat
10th Annual meeting of the Society for Bimolecular Sciences (2004)

・ Development and Evaluation of the New HTRF-reader “Artemis”
K. Ukegawa, C. Tokuda, S. Ito, T. Iketani
12th Annual meeting of the Society for Bimolecular Sciences (2006)

・Development of an SIRT1 Deacetylase Assay using HTRF® Technology
C. Tokuda, F. Chevallier and M. Preauda
13th Annual meeting of the Society for Bimolecular Sciences (2007)

・New Apolipoprotein A1 and Apolipoprotein B assay development using HTRF® Technology
K. Adachi and C. Tokuda
15th Annual meeting of the Society for Bimolecular Sciences (2009)

・Up- And Down-Regulation Caused By RXR Agonist And HMG-CoA Reductase Inhibitor are Detectable With HTRFョ Apo-A1 And Apo-B Assays.
K. Adachi, C. Tokuda
16th Annual meeting of the Society for Bimolecular Sciences (2010)

・Measurement of Glucagon-Like Peptide 1 With the HTRF ® Assay
C. Tokuda, K. Adachi, M. Preaudat
17th Annual meeting of the Society for Bimolecular Sciences (2011)

・Development of G9a(Histone H3K9 methyltransferase) assay using HTRF® Technology
K. Adachi, C. Tokuda, F. Chevallier and M. Preauda
17th Annual meeting of the Society for Bimolecular Sciences (2011)

・High-Throughput, Homogeneous Histone H3 Methyltrasferase (HMT) and Demethylase (HDM) Enzyme assays using HTRF Technology
K. Adachi, C. Tokuda , T. Roux, F. Chevallier , A. Gonzalwz-Moya
Discovery on Target (2012)

・High-Throughput, Homogeneous Histone Demethylase JARID1A and JARID1C Enzymatic applications with HTRF Technology
K. Adachi, C. Tokuda , T. Roux, E. Trinquet
16th MipTec(2013) Poster award

・創薬研究におけるHTRF技術
細谷純子、徳田千賀志
医薬ジャーナル 新薬展望2006 (Vol.42 S-1)

・HTRF(ホモジニアス時間分解蛍光)法によるGPCR研究
徳田千賀志
Clinical Neuroscience Vol.32 (2014)

 

 

HTRF®アッセイ開発のコツ

2017年3月 スクリーニング学研究会チュートリアルにて

弊社
シニアサイエンティフィック アドバイザー
徳田 千賀志

発表内容: HTRF法の原理とアッセイ系開発のコツ


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